Table 1.
Telo-FISH analysis of two mouse cell lines treated with inhibitor of DNA-PKcs
| |
Frequency (event/cell) |
|||
|---|---|---|---|---|
| Cell Line | Metaphases Scored | RB fusion | Telomeric Fusions | Breaks/Fragments |
|
LY-R |
|
|
|
|
| Untreated (DMSO) |
213 |
0.037b ± 0.012 |
0.075b ± 0.021 |
0.056 |
| Treated (DNA-PKcsi) |
201 |
0.088a ± 0.048 |
0.279a ± 0.011 |
0.277 |
|
LY-S |
|
|
|
|
| Untreated (DMSO) |
189 |
0.159b ± 0.025 |
0.126b ± 0.003 |
0.140 |
| Treated (DNA-PKcsi) | 200 | 0.249a ± 0.046 | 0.410a ± 0.027 | 0.212 |
Frequency of Robertsonian fusion, end-to-end telomeric fusions and DNA-DSB/fragments were recorded from two independent experiments in the LY-R and LY-S cell lines. The inhibition was induced for 24 -hour period before metaphase preparation. DMSO was used as control treatment. In each experiment at least 100 metaphases were scored (except in LY-S untreated). S.D. was used as measure of dispersion from the mean.
a The difference in mean event/cell between untreated and treated cell lines are significant (p < 0.05).
b The difference in mean event/cell between untreated cell lines are significant (p < 0.0001).