Table 1.
Analytical properties of the rabbit polyclonal antibodies
| Hapten | Protein carrier | Coupling method | Titer (RIA) | Affinity (M-1) | Specificity (%) |
|
|---|---|---|---|---|---|---|
| Benzoyl-ecgonine | Cocaethylene | |||||
| 2 | BSA | EDC | 25-669 | 3.5-5.0 × 108 | >100 | 96 |
| 3 | BSA | EDC | 318-450 | 0.8-1.6 × 109 | 82-90 | >100 |
| BSA | MA | 221-2682 | 1.5-9.7 × 108 | 0.3-10 | 35-59 | |
| KLH | EDC | 95-325 | 5.2-6.9 × 109 | 40-54 | >100 | |
| KLH | MA | 631-1653 | 1.5-3.8 × 109 | 1-13 | 23-31 | |
| 4 | BSA | EDC | No | significant | response | |
| KLH | EDC | |||||
| 5a | BSA | MA | 7790-12832 | 1-1.6 × 109 | 45-62 | 57-58 |
| KLH | MA | 79-198 | 6.2-9.2 × 109 | 100 | 67-100 | |
| 5b | BSA | MA | 755-3382 | 1.5-5.9 × 1011 | 2.6-17 | 71-100 |
| KLH | MA | 165-1408 | 1.4-1.7 × 109 | 0.8-3 | 55-147 | |
| TT | MA | 277-940 | 1.2-5.3 × 1010 | 18-23 | 95-99 | |
| 6 | BSA | MA | 508-1891 | 2.2-8.5 × 1010 | 13-16 | 12-75 |
| TT | MA | 24-36 | 0.3-2.3 × 1011 | 0.8-1.2 | 18-86 | |
BSA, Bovine Serum Albumin; KLH, Keyhole Limpet Hemocyanin; TT, Tetanus Toxoid; EDC, 1-Ethyl-3-[3-dimethylaminopropyl]-carbodiimide hydrochloride; MA, mixed anhydride.