Table 6.
Application of the proposed methods for the determination of the studied drugs in their raw materials
| Compound | Proposed methods |
Reference methods (30, 31) |
|||
|---|---|---|---|---|---|
| Amount taken (μg/ml) | Amount found (μg/ml) | Recovery (%) | Amount taken (μg/ml) | Recovery (%) | |
| At 610 nm | |||||
| Acarbose | 4.0 | 3.978 | 99.45 | ||
| 5.0 | 4.919 | 98.38 | |||
| 10.0 | 10.135 | 101.35 | |||
| 15.0 | 15.039 | 100.26 | |||
| 20.0 | 19.926 | 99.63 | |||
| x ± S.D. | 99.81 ± 1.093 | 99.20 ± 0.400 | |||
| t | 0.906 | (2.447)a | |||
| F | 7.47 | (19.25) | |||
| Miglitol | 1.0 | 1.021 | 102.10 | ||
| 2.0 | 2.015 | 100.75 | 60.0 | 101.00 | |
| 4.0 | 3.937 | 98.43 | 80.0 | 98.50 | |
| 6.0 | 6.019 | 100.32 | 100.0 | 100.61 | |
| 8.0 | 8.000 | 100.00 | |||
| 10.0 | 10.009 | 100.09 | |||
| x ± S.D. | 100.28 ± 1.189 | 100.04 ± 1.350 | |||
| t | 0.274 | (2.365)a | |||
| F | 1.29 | (19.30) | |||
| At 525 nm | |||||
| Acarbose | 4.0 | 4.039 | 100.98 | ||
| 5.0 | 4.950 | 99.00 | |||
| 100 | 10.027 | 100.27 | |||
| 15.0 | 14.971 | 99.81 | |||
| 20.0 | 20.013 | 100.06 | |||
| x ± S.D. | 100.02 ± 0.719 | 99.20 ± 0.400 | |||
| t | 1.779 | (2.447)a | |||
| F | 3.23 | (19.25) | |||
| Miglitol | 2.0 | 1.967 | 98.35 | ||
| 4.0 | 4.045 | 101.13 | 60.0 | 101.00 | |
| 6.0 | 6.067 | 101.12 | 80.0 | 98.50 | |
| 8.0 | 8.052 | 100.65 | 100.0 | 100.61 | |
| 10.0 | 9.911 | 99.11 | |||
| x ± S.D. | 100.07 ± 1.270 | 100.04 ± 1.350 | |||
| t | 0.032 | (2.447)a | |||
| F | 1.13 | (19.25) | |||
Each result is the average of three separate determinations.
Figures in parentheses are the tabulated t and F values, respectively at p=0.05 (32).