Figure 3.

Effect of mycoplasmas on cell growth. Mycoplasma-transformed and control 32D cells cultured in RPMI 1640 medium containing 10% FBS and IL-3 at least for 3 weeks were harvested by centrifugation and then transferred into fresh medium with (+) or without IL-3 (-) immediately followed by adding mycoplasmal stocks into the cultures. The same amount of SP4 medium was added into non infected control culture. The viability of the cells at set time points was examined by the trypan blue exclusion assay. The results represent 3 similar experiments.