. 2007 Dec;3(4):269–278.

Table 1.

Preliminary screening of secondary metabolites from callus of S. febrifuga

Tests for Secondary metabolites		Hexane	Ethyl acetate	Methanol

Alkaloids	Mayers test	-	-	-
	Wagners test	-	-	-
	Dragendorff’s test	-	-	-
Flavonoids	FeCl₃ test	+	+	+
	Shinoda’s test	+	+	+
	Zinc HCl reduction test	+	+	+
	Lead acetate	+	+	+
Phenols	Phenol test	-	+	+
Phenols	Ellagic test	-	+	+
Glycosides	Kellar Kilani test	-	+	-
Lignin	Labat test	-	-	-
Lignin	Lignin test	-	-	-
Tannins	Gelatin test	+	+	+
	FeCl₃ test	+	+	+
	Lead acetate	-	-	-
Terpenoids		-	+	----
Quinones		----	+	----
Saponins		-	+	-

+, indicates presence; -, absence.