Figure 4.

Expression levels of survivin are independent of irradiation-induced apoptosis. The two survivin expressing cell lines B16-F10 and MO5 were irradiated for the induction of apoptosis. Aliquots of non-irradiated and irradiated cells were washed and lysed in RIPA buffer supplemented with a protease inhibitor cocktail. Equal amounts of protein (10 μg) were subjected to SDS-PAGE and Western blot analysis with anti-survivin and anti-tubulin antibodies. All four samples contained approximately equal amounts of protein as confirmed by the anti-tubulin labelling, and expressed comparable amounts of survivin, indicating that the survivin expression levels were independent of induction of apoptosis.