Table 1.
Chemokines in experimental MI models
| Model | Species | Chemokine | Detection | Expression site | Time (Peak) | References |
|---|---|---|---|---|---|---|
| AMI | Mouse | MCP-1 | RPA | Non-infarct | Day 1, 3, 7, 28 | 9 |
| AMI | Mouse | SDF-1 | PCR ELISA | Infarcted myocardium | 48, 72, 96 h | 19 |
| AMI | Mouse | SDF-1 | RT-PCR WB | Infarcted heart | 0.5, 1, 2, 4, 8, 16 day | 20 |
| AMI | Rat | SDF-1a | NB | Left ventricle | 0.25h, 1h, 24h, 1w, 3w, 6 week | 21 |
| AMI | Rat | MCP-1f IL-8 | NB | Infarcted area | 1, 6 week | 15 |
| AMI | Rat | IL-8 GRO-α | TR-PCR ELISA | Ischemic heart Serum | 6, 12, 24, 48 h | 16 |
| AMI | Rat | MCP-1 | ISH IHC | Infarcted area | Day 3, 7, 14, 21 | 24 |
| I/R | Mouse | MIP-1α MIP-1β MIP-2 | RPA IHC | Myocardium | 15 min I + 3hR | 26 |
| I/R | Mouse | MCP-1 MIP-1α MIP-2 | RT-PCR | Ischemic heart | 30 min I + 120 min R | 23 |
| I/R | Rat | MCP-1 | NB | Ischemic reperfused myocardium | 25 min I + 2 h R | 22 |
| I/R | Rabbit | IL-8 | RIA | Ischemic myocardium | 45 min I + 1.5, 3, 4.5 h R | 17 |
| I/R | Rabbit | IL-8 | ELISA | Ischemic heart | 1, 2, 4, 8 h | 18 |
| I/R | Dog | MCP-1 | NB ISH | Reperfused area | 1, 3, 5, 24, 48 h | 7 |
| I/R | Dog | MCP-1 | RPA ISH | Ischemic area | 15min I + 5 h R | 25 |
AMI, acute myocardial infarction; I, ischemia; R, reperfusion; RPA, ribonuclease protection assay; PCR, polymerase chain reaction; RTPCR, reverse transcriptase-PCR; WB, western blot; NB, northern blot; ELISA, enzyme-linked immunosorbent assay; ISH, in situ hybridyzation; IHC, immunohistochemitry; RIA, raioimmunoassay.