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. 2008 Sep;4(3):179–184.

Figure 5.

Figure 5

Melting curve analysis by real time PCR. Derivatives, displays a plot of the first derivative of the rate of change in fluorescence as a function of temperature; 1, negative (H2O); 2-5, positive with NG1 and NG2 primer specific for genus Nocardia; 6, M. tuberculosis H37Rv; 7, DNA from M. tuberculosis.