Table 3.

Application of the proposed and official methods to the determination of biotin in pure form

Compound	Proposed method			Official method (3)
	Concentration taken (μg/mL)	Concentration found (μg/mL)	Recovery (%)	Recovery (%)
Biotin	4.0	3.976	99.40	98.04
	5.0	4.979	99.58	100.98
	6.0	6.090	101.50	98.04
	8.0	8.026	100.33
	10.0	9.997	99.97
	12.0	11.968	99.73
	14.0	13.940	99.57
	16.0	16.090	100.56
Mean ± S.D			100.08 ± 0.761	99.02 ± 1.69
Student’s t-test			1.42 (2.26)
F-test			5.95 (19.35)

Each result is the average of three separate determinations. Values in parentheses are the tabulated t and F values, respectively at p=0.05 (40).