Table 5.

Application of the proposed method to the determination of biotin in its pharmaceutical preparations

Compound	Proposed method			Official method (3)
	Concentration taken (μg/mL)	Concentration found (μg/mL)	Recovery (%)	Recovery (%)
Biotine Bayer 0.5% inja	8.0	8.200	102.50	102.09
(Biotin 5 mg/mL)	10.0	10.222	102.22	99.98
Batch # F0120	12.0	11.857	98.81	100.70
Mean ± S. D			101.17 ± 2.05	100.92 ± 1.07
% RSD			2.030	1.062
%Error			1.172	0.613
Student’s t-test			0.11 (2.78)
F-test			3.67 (19.00)
Biotin forte capsulesb	6.0	5.965	99.42	98.96
(Biotin 5 mg/capsule)	8.0	7.822	97.78	99.93
Batch # 7433017	12.0	11.570	96.42	99.45
Mean ± S. D			97.87 ± 1.50	99.44 ± 0.48
% RSD			1.534	0.487
%Error			0.886	0.281
Student’s t-test			1.73 (2.78)
F-test			9.59 (19.00)

Each result is the average of three separate determinations. Values in parentheses are the tabulated t and F values, respectively at p=0.05 (40).

^aProduct of Bayer Sante Familiale, Les Moulineaux Cedex 9, France;

^bProduct of Unipharma, El Obour City, Cairo, Egypt.