Figure 4.

NF-kB activation by TNFα or IL-1β in MG-63 cells in the presence of NAC. MG-63 nuclear extracts were analysed by EMSA in the presence of IL-6κB radioactive probe. Cells were induced with cytokines (3-8 IL-1β, 9-14 TNFα) for 30 min, one hour after addition of NAC (6, 12 15 mM, 7, 13 30 mM, 8,14 50mM). To check binding specificity, 100 fold excess of IL-6κB cold oligonucleotide was added to lanes 5 and 11. Complexes of NF-κB heterodimer, p50 dimer or CBF1 are indicated by asterisk, arrowhead or circle, respectively. The data shown in the figure are representative of three independent experiments.