Figure 2.

Elution profile of HDL in PD-10 columns. HDL was dialyzed in PBS by gel filtration chromatography using PD-10 columns. Four hundred μL of 3 different HDL samples were fractionated in a PD-10 column, as described in Methods. Seven aliquots of 200 μL were collected, as described in Methods, and apoA-I concentration was measured. The table shows the mg of apoA-I in each aliquot. The recovery of fractions 3 to 6 is indicated in the last column.