Table 2.

Application of the proposed method and comparison method for the determination of NF and TZ in pure state

Drug determined	Proposed method					Reference method (34, 38)
	Amount taken (μg/mL)	Amount found (μg/mL)	% Found	% RSD	ΔЄ (Mole-1. L-1)	Amount taken (μg/mL)	% Found
	2	1.99	99.50	0.85	10538.22	4	100.25
	4	3.93	98.25	0.84	11097.07	6	98.83
	6	6.07	101.17	0.94	11709.24	8	101.63
	8	7.93	99.13	0.79	11576.08	10	99.00
	10	10.06	100.6	0.96	11815.58	12	100.17
	12	11.99	99.92	0.99	11789.07
	14	13.98	99.86	0.80	11815.58
	16	16.19	101.19	0.88	11995.37
	18	18.02	100.11	0.89	11886.47
	20	19.85	99.25	0.94	11799.61
	% R ± SD = 99.90 ± 0.93					% R ± SD = 99.98 ± 1.13
	t-value = 0.19 (2.16)
	F-value = 1.49 (3.63)
	5	5.09	101.80	0.89	4203.59	6	100.00
	10	10.07	100.70	0.85	3931.62	9	99.33
	15	15.05	100.33	0.94	3840.88	12	100.58
	20	19.95	99.75	0.91	3783.26	15	100.13
	25	25.00	100.00	0.71	3768.43	18	99.78
	30	29.84	99.47	0.77	3733.81
	35	34.68	99.09	0.86	3709.08
	40	40.06	100.15	0.80	3739.99
	45	45.04	100.09	0.78	3731.09
	50	50.22	100.44	0.90	3738.75
	% R ± SD = 100.18 ± 0.74					% R ± SD = 99.96 ± 0.46
	t-value = 0.62 (2.16)
	F-value = 2.58 (6)

Figures between brackets are the tabulated t and F-values at (P=0.05). ΔЄ is the molar absorbativity. % R is the mean value of percent recovery. % RSD is calculated using the results of three separate determinations for each concentration.