Table 1. Proteomic profile of membrane protein distribution in sorted populations of reticulocytes and extruded nuclei.
| Nuclei | Reticulocytes | ||||
| Accession | Description | Total peptides | Unique peptides | Total peptides | Unique peptides |
| Q9HDC9 | Adipocyte plasma membrane-associated protein | 22 | 12 | 6 | 4 |
| Q02094 | Ammonium transporter Rh type A | 3 | 2 | 6 | 2 |
| B4DNW4 | Aquaporin 1 | 9 | 3 | 12 | 4 |
| Q5T5M0 | Aquaporin 7 | 4 | 1 | 3 | 1 |
| Q9NP58 | ATP-binding cassette sub-family B member 6, mitochondrial | 4 | 4 | 40 | 18 |
| P02730 | Band 3 anion transport protein | 195 | 28 | 477 | 36 |
| Q54A51 | Basigin | 36 | 8 | 32 | 8 |
| B6EAT9 | CD44 | 2 | 2 | 4 | 2 |
| E9PB22 | CD47 | 3 | 1 | ||
| Q99808 | Equilibrative nucleoside transporter 1 | 11 | 5 | 14 | 5 |
| Q96PL5 | Erythroid membrane-associated protein | 14 | 7 | ||
| O75955 | Flotillin-1 | 9 | 8 | 46 | 20 |
| Q14254 | Flotillin-2 | 6 | 5 | 42 | 20 |
| P11166 | Glucose transporter, type 1 | 51 | 10 | 78 | 12 |
| P04921 | Glycophorin-C | 13 | 2 | 29 | 3 |
| Q86SU0 | Immunoglobulin-like domain-containing receptor 1 | 9 | 1 | 1 | 1 |
| P20702 | Integrin alpha-X | 6 | 2 | 1 | 1 |
| P05556 | Integrin beta-1 | 11 | 7 | 2 | 2 |
| P23276 | Kell blood group glycoprotein | 6 | 4 | ||
| O75387 | Large neutral amino acids transporter small subunit 3 | 2 | 1 | 4 | 2 |
| P51811 | Membrane transport protein XK | 4 | 3 | ||
| O15173 | Membrane-associated progesterone receptor component 2 | 25 | 6 | 4 | 2 |
| P53985 | Monocarboxylate transporter 1 | 7 | 4 | 6 | 4 |
| O15439 | Multidrug resistance-associated protein 4 | 2 | 2 | 12 | 10 |
| Q6PIU2 | Neutral cholesterol ester hydrolase 1 | 52 | 16 | 12 | 7 |
| P20020 | Plasma membrane calcium-transporting ATPase 1 | 7 | 5 | 14 | 11 |
| Q16720 | Plasma membrane calcium-transporting ATPase 3 | 6 | 4 | 9 | 7 |
| P23634 | Plasma membrane calcium-transporting ATPase 4 | 12 | 10 | 20 | 16 |
| Q9Y4D8 | Probable E3 ubiquitin-protein ligase C12orf51 | 1 | 1 | 44 | 33 |
| Q5VSJ9 | Rh blood group, CcEe antigens | 3 | 2 | 8 | 4 |
| E9PS74 | SLC43A3 | 3 | 2 | 4 | 3 |
| Q96QG1 | Sodium/calcium exchanger SCL8A3 | 2 | 2 | ||
| B7Z3U6 | Sodium/potassium-transporting ATPase subunit alpha-1 | 14 | 9 | 14 | 12 |
| P54709 | Sodium/potassium-transporting ATPase subunit beta-3 | 4 | 3 | 2 | 2 |
| P27105 | Stomatin | 118 | 16 | 126 | 16 |
| Q9UJZ1 | Stomatin-like protein 2 | 5 | 4 | 13 | 11 |
| Q9H1E5 | Thioredoxin-related transmembrane protein 4 | 3 | 2 | ||
| A6NJC0 | TMCC2 | 65 | 13 | 18 | 6 |
| P02786 | Transferrin receptor protein 1 | 81 | 26 | 75 | 25 |
| B7Z1P7 | Transmembrane and coiled-coil domain family 2 | 151 | 25 | 36 | 15 |
| Q13336 | Urea transporter 1 | 2 | 2 | ||
| Q9Y6M5 | Zinc transporter 1 | 2 | 1 | 4 | 4 |
Sorted populations of reticulocytes and extruded nuclei were fractionated by 1D SDS-PAGE and subjected to Nano LC mass spectrometry. An abridged list containing key erythroid membrane proteins of interest is shown. Total peptide column is the total number of peptides (and therefore an indication of a particular protein’s abundance) detected in the population, whilst the unique peptide column indicates the number of unique peptides detected. To assess differences between nuclei and reticulocyte populations the total peptide number should be used.