Table 2. Proteomic profile of erythroid cytoskeletal protein distribution in sorted populations of reticulocytes and extruded nuclei.
| Nuclei | Reticulocytes | ||||
| Accession | Description | Total peptides | Unique peptides | Total peptides | Unique peptides |
| Q00013 | 55 kDa erythrocyte membrane protein | 27 | 15 | 93 | 27 |
| P68032 | Actin, alpha cardiac muscle | 116 | 16 | 127 | 15 |
| P60709 | Actin, cytoplasmic | 223 | 25 | 228 | 24 |
| P61160 | Actin-related protein 2 | 6 | 5 | 22 | 11 |
| O15143 | Actin-related protein 2/3 complex subunit 1B | 2 | 2 | 14 | 7 |
| O15144 | Actin-related protein 2/3 complex subunit 2 | 4 | 3 | 25 | 11 |
| P61158 | Actin-related protein 3 | 12 | 9 | 44 | 15 |
| O43707 | Alpha-actinin-4 | 4 | 4 | 12 | 7 |
| P35611 | Alpha-adducin | 9 | 5 | 78 | 20 |
| P16157 | Ankyrin-1 | 123 | 65 | 476 | 104 |
| E9PE32 | Ankyrin-3 | 8 | 5 | 31 | 9 |
| Q562R1 | Beta-actin-like protein 2 | 41 | 6 | 46 | 6 |
| P35612 | Beta-adducin | 72 | 25 | ||
| B1AK87 | Capping protein (Actin filament) muscle Z-line, beta | 10 | 4 | 43 | 12 |
| Q96H99 | Cortactin | 1 | 1 | 20 | 13 |
| Q08495 | Dematin | 16 | 10 | 83 | 21 |
| A8K8J9 | Dynactin 2 (P50), isoform CRA_b | 6 | 5 | 32 | 16 |
| Q4KKX0 | Erythrocyte membrane protein band 4.2 | 30 | 13 | 164 | 40 |
| P21333 | Filamin-A | 27 | 23 | 94 | 66 |
| Q9UEY8 | Gamma-adducin | 14 | 7 | ||
| A2A418 | Gelsolin | 23 | 9 | 15 | 7 |
| P33176 | Kinesin-1 heavy chain | 17 | 12 | ||
| Q15691 | Microtubule-associated protein RP/EB family member 1 | 5 | 3 | 12 | 8 |
| P12829 | Myosin light chain 4 | 10 | 5 | 24 | 8 |
| P60660 | Myosin light polypeptide 6 | 3 | 1 | 4 | 3 |
| Q3MIV8 | Myosin, heavy chain 11, smooth muscle | 12 | 9 | 43 | 19 |
| P35580 | Myosin-10 | 48 | 38 | 210 | 106 |
| Q7Z406 | Myosin-14 | 10 | 8 | 25 | 12 |
| P35579 | Myosin-9 | 75 | 54 | 373 | 124 |
| P11171 | Protein 4.1 | 38 | 17 | 159 | 33 |
| P02549 | Spectrin alpha chain, erythrocyte | 138 | 87 | 735 | 176 |
| P11277 | Spectrin beta chain, erythrocyte | 105 | 70 | 637 | 165 |
| Q9Y490 | Talin-1 | 66 | 48 | 224 | 102 |
| Q9Y4G6 | Talin-2 | 6 | 6 | 24 | 11 |
| P28289 | Tropomodulin-1 | 4 | 4 | 32 | 16 |
| D9YZV5 | Tropomyosin 1 (Alpha) isoform 4 | 9 | 5 | 10 | 4 |
| Q5VU58 | Tropomyosin 3 | 19 | 9 | 37 | 12 |
| P06753 | Tropomyosin alpha-3 chain | 11 | 6 | 18 | 6 |
| Q71U36 | Tubulin alpha-1 chain | 61 | 13 | 141 | 21 |
| P07437 | Tubulin beta chain | 89 | 23 | 249 | 28 |
| A8MUB1 | Tubulin, alpha 1 (Testis specific) | 49 | 9 | 119 | 20 |
| B3KPW9 | Tubulin, alpha 8 | 33 | 7 | 80 | 13 |
| B3KS31 | Tubulin, beta 6 | 23 | 7 | 61 | 9 |
| P18206 | Vinculin | 9 | 6 | 57 | 35 |
Sorted populations of reticulocytes and extruded nuclei were fractionated by 1D SDS-PAGE and subjected to Nano LC mass spectrometry. An abridged list containing key cytoskeletal proteins of interest is shown. Total peptide column is the total number of peptides (and therefore an indication of a particular protein’s abundance) detected in the population, whilst the unique peptide column indicates the number of unique peptides detected. To assess differences between nuclei and reticulocyte populations the total peptide number should be used.