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. 2013 Apr 2;8(4):e60300. doi: 10.1371/journal.pone.0060300

Table 4. Proteomic profile of cytosolic and endocytic protein distribution in sorted populations of reticulocytes and extruded nuclei.

Nuclei Reticulocytes
Accession Description Total peptides Unique peptides Total peptides Unique peptides
P62258 14-3-3 protein epsilon 43 16 85 20
P61981 14-3-3 protein gamma 26 12 34 11
P63104 14-3-3 protein zeta/delta 35 12 50 11
Q01813 6-phosphofructokinase type C 1 1 47 21
B4DQJ8 6-phosphogluconate dehydrogenase, decarboxylating 24 12 84 24
P49588 Alanyl-tRNA synthetase, cytoplasmic 13 10 93 37
Q10567 AP-1 complex subunit beta-1 3 3 28 21
O95782 AP-2 complex subunit alpha-1 6 6 69 32
P63010 AP-2 complex subunit beta 6 5 46 27
Q2M2I8 AP2-associated protein kinase 1 5 5
C9JPM4 ARF4 23 6 7 4
P53396 ATP-citrate synthase 26 16 166 49
P07738 Bisphosphoglycerate mutase 34 13 78 17
P11586 C-1-tetrahydrofolate synthase, cytoplasmic 26 17 141 48
P07384 Calpain-1 catalytic subunit 7 6 78 36
P00915 Carbonic anhydrase 1 32 11 64 14
P00918 Carbonic anhydrase 2 49 15 92 21
P04040 Catalase 95 32 376 47
Q00610 Clathrin heavy chain 1 49 35 243 80
P53675 Clathrin heavy chain 2 6 6 40 17
P30046 D-dopachrome decarboxylase 1 1 7 5
Q16531 DNA damage-binding protein 1 41 22 89 48
P46734 Dual specificity mitogen-activated protein kinase kinase 3 33 15 57 20
P50570 Dynamin-2 3 3 23 16
E9PD66 E3 ubiquitin-protein ligase HUWE1 12 7 118 84
Q15075 Early endosome antigen 1 2 2 1 1
P13639 Elongation factor 2 23 11 84 32
P60842 Eukaryotic initiation factor 4A-I 52 18 90 23
P49327 Fatty acid synthase 19 15 135 76
P30043 Flavin reductase 123 16 253 19
P04075 Fructose-bisphosphate aldolase A 55 20 181 31
P11413 Glucose-6-phosphate 1-dehydrogenase 13 9 84 31
P48506 Glutamate–cysteine ligase catalytic subunit 4 4 81 31
E7EU54 Glyceraldehyde-3-phosphate dehydrogenase 42 11 104 14
P49840 Glycogen synthase kinase-3 alpha 3 3 7 4
P08107 Heat shock 70 kDa protein 1A/1B 77 23 96 32
P34932 Heat shock 70 kDa protein 4 10 7 77 35
P17066 Heat shock 70 kDa protein 6 39 9 35 8
P07900 Heat shock protein HSP 90-alpha 114 35 177 45
P08238 Heat shock protein HSP 90-beta 77 31 94 33
P54652 Heat shock-related 70 kDa protein 2 61 11 54 12
P69905 Hemoglobin subunit alpha 532 14 713 17
P68871 Hemoglobin subunit beta 802 20 1199 21
P07195 L-lactate dehydrogenase B chain 35 14 118 23
P11279 Lysosome-associated membrane glycoprotein 1 3 2 10 5
P32119 Peroxiredoxin-2 107 18 282 18
P30041 Peroxiredoxin-6 41 13 95 18
Q13492 Phosphatidylinositol-binding clathrin assembly protein 1 1 18 13
P00558 Phosphoglycerate kinase 1 46 20 102 28
F2Z2J9 Phosphoglycerate mutase 1 1 55 15
P08397 Porphobilinogen deaminase 55 18 111 22
Q9UKV8 Protein argonaute-2 6 4 43 22
P00491 Purine nucleoside phosphorylase 67 16 141 21
P30613 Pyruvate kinase isozymes R/L 17 13 97 32
P50395 Rab GDP dissociation inhibitor beta 54 28 132 40
Q96NA2 Rab-interacting lysosomal protein 2 2 27 14
Q99986 Serine/threonine-protein kinase VRK1 41 20
F5GWT4 Serine/threonine-protein kinase WNK1 1 1 24 20
A6NKH4 Sorting nexin 1 8 7
B4DEK4 Sorting nexin 2 1 1 16 13
Q9NRS6 Sorting nexin-15 3 3
Q9Y5X3 Sorting nexin-5 1 1 4 4
Q9UNH7 Sorting nexin-6 2 1 10 9
Q9Y5X1 Sorting nexin-9 4 3
Q9H2G2 STE20-like serine/threonine-protein kinase 1 1 22 16
P31948 Stress-induced-phosphoprotein 1 28 17 101 39
P17987 T-complex protein 1 subunit alpha 43 22 122 31
P37837 Transaldolase 44 20 74 26
P29401 Transketolase 42 14 117 33
P60174 Triosephosphate isomerase 44 15 104 23
P29144 Tripeptidyl-peptidase 2 1 1 78 45
P54578 Ubiquitin carboxyl-terminal hydrolase 14 18 9 87 27
Q9C0C9 Ubiquitin-conjugating enzyme E2 O 5 3 77 37
Q96RL7 Vacuolar protein sorting-associated protein 13A 11 9
F5GYF5 Vacuolar protein sorting-associated protein 35 1 1 14 11

Sorted populations of reticulocytes and extruded nuclei were fractionated by 1D SDS-PAGE and subjected to Nano LC mass spectrometry. An abridged list containing key cytosolic and endocytic proteins of interest is shown. Total peptide column is the total number of peptides (and therefore an indication of a particular protein’s abundance) detected in the population, whilst the unique peptide column indicates the number of unique peptides detected. To assess differences between nuclei and reticulocyte populations the total peptide number should be used.