Table 4. Proteomic profile of cytosolic and endocytic protein distribution in sorted populations of reticulocytes and extruded nuclei.
Nuclei | Reticulocytes | ||||
Accession | Description | Total peptides | Unique peptides | Total peptides | Unique peptides |
P62258 | 14-3-3 protein epsilon | 43 | 16 | 85 | 20 |
P61981 | 14-3-3 protein gamma | 26 | 12 | 34 | 11 |
P63104 | 14-3-3 protein zeta/delta | 35 | 12 | 50 | 11 |
Q01813 | 6-phosphofructokinase type C | 1 | 1 | 47 | 21 |
B4DQJ8 | 6-phosphogluconate dehydrogenase, decarboxylating | 24 | 12 | 84 | 24 |
P49588 | Alanyl-tRNA synthetase, cytoplasmic | 13 | 10 | 93 | 37 |
Q10567 | AP-1 complex subunit beta-1 | 3 | 3 | 28 | 21 |
O95782 | AP-2 complex subunit alpha-1 | 6 | 6 | 69 | 32 |
P63010 | AP-2 complex subunit beta | 6 | 5 | 46 | 27 |
Q2M2I8 | AP2-associated protein kinase 1 | 5 | 5 | ||
C9JPM4 | ARF4 | 23 | 6 | 7 | 4 |
P53396 | ATP-citrate synthase | 26 | 16 | 166 | 49 |
P07738 | Bisphosphoglycerate mutase | 34 | 13 | 78 | 17 |
P11586 | C-1-tetrahydrofolate synthase, cytoplasmic | 26 | 17 | 141 | 48 |
P07384 | Calpain-1 catalytic subunit | 7 | 6 | 78 | 36 |
P00915 | Carbonic anhydrase 1 | 32 | 11 | 64 | 14 |
P00918 | Carbonic anhydrase 2 | 49 | 15 | 92 | 21 |
P04040 | Catalase | 95 | 32 | 376 | 47 |
Q00610 | Clathrin heavy chain 1 | 49 | 35 | 243 | 80 |
P53675 | Clathrin heavy chain 2 | 6 | 6 | 40 | 17 |
P30046 | D-dopachrome decarboxylase | 1 | 1 | 7 | 5 |
Q16531 | DNA damage-binding protein 1 | 41 | 22 | 89 | 48 |
P46734 | Dual specificity mitogen-activated protein kinase kinase 3 | 33 | 15 | 57 | 20 |
P50570 | Dynamin-2 | 3 | 3 | 23 | 16 |
E9PD66 | E3 ubiquitin-protein ligase HUWE1 | 12 | 7 | 118 | 84 |
Q15075 | Early endosome antigen 1 | 2 | 2 | 1 | 1 |
P13639 | Elongation factor 2 | 23 | 11 | 84 | 32 |
P60842 | Eukaryotic initiation factor 4A-I | 52 | 18 | 90 | 23 |
P49327 | Fatty acid synthase | 19 | 15 | 135 | 76 |
P30043 | Flavin reductase | 123 | 16 | 253 | 19 |
P04075 | Fructose-bisphosphate aldolase A | 55 | 20 | 181 | 31 |
P11413 | Glucose-6-phosphate 1-dehydrogenase | 13 | 9 | 84 | 31 |
P48506 | Glutamate–cysteine ligase catalytic subunit | 4 | 4 | 81 | 31 |
E7EU54 | Glyceraldehyde-3-phosphate dehydrogenase | 42 | 11 | 104 | 14 |
P49840 | Glycogen synthase kinase-3 alpha | 3 | 3 | 7 | 4 |
P08107 | Heat shock 70 kDa protein 1A/1B | 77 | 23 | 96 | 32 |
P34932 | Heat shock 70 kDa protein 4 | 10 | 7 | 77 | 35 |
P17066 | Heat shock 70 kDa protein 6 | 39 | 9 | 35 | 8 |
P07900 | Heat shock protein HSP 90-alpha | 114 | 35 | 177 | 45 |
P08238 | Heat shock protein HSP 90-beta | 77 | 31 | 94 | 33 |
P54652 | Heat shock-related 70 kDa protein 2 | 61 | 11 | 54 | 12 |
P69905 | Hemoglobin subunit alpha | 532 | 14 | 713 | 17 |
P68871 | Hemoglobin subunit beta | 802 | 20 | 1199 | 21 |
P07195 | L-lactate dehydrogenase B chain | 35 | 14 | 118 | 23 |
P11279 | Lysosome-associated membrane glycoprotein 1 | 3 | 2 | 10 | 5 |
P32119 | Peroxiredoxin-2 | 107 | 18 | 282 | 18 |
P30041 | Peroxiredoxin-6 | 41 | 13 | 95 | 18 |
Q13492 | Phosphatidylinositol-binding clathrin assembly protein | 1 | 1 | 18 | 13 |
P00558 | Phosphoglycerate kinase 1 | 46 | 20 | 102 | 28 |
F2Z2J9 | Phosphoglycerate mutase | 1 | 1 | 55 | 15 |
P08397 | Porphobilinogen deaminase | 55 | 18 | 111 | 22 |
Q9UKV8 | Protein argonaute-2 | 6 | 4 | 43 | 22 |
P00491 | Purine nucleoside phosphorylase | 67 | 16 | 141 | 21 |
P30613 | Pyruvate kinase isozymes R/L | 17 | 13 | 97 | 32 |
P50395 | Rab GDP dissociation inhibitor beta | 54 | 28 | 132 | 40 |
Q96NA2 | Rab-interacting lysosomal protein | 2 | 2 | 27 | 14 |
Q99986 | Serine/threonine-protein kinase VRK1 | 41 | 20 | ||
F5GWT4 | Serine/threonine-protein kinase WNK1 | 1 | 1 | 24 | 20 |
A6NKH4 | Sorting nexin 1 | 8 | 7 | ||
B4DEK4 | Sorting nexin 2 | 1 | 1 | 16 | 13 |
Q9NRS6 | Sorting nexin-15 | 3 | 3 | ||
Q9Y5X3 | Sorting nexin-5 | 1 | 1 | 4 | 4 |
Q9UNH7 | Sorting nexin-6 | 2 | 1 | 10 | 9 |
Q9Y5X1 | Sorting nexin-9 | 4 | 3 | ||
Q9H2G2 | STE20-like serine/threonine-protein kinase | 1 | 1 | 22 | 16 |
P31948 | Stress-induced-phosphoprotein 1 | 28 | 17 | 101 | 39 |
P17987 | T-complex protein 1 subunit alpha | 43 | 22 | 122 | 31 |
P37837 | Transaldolase | 44 | 20 | 74 | 26 |
P29401 | Transketolase | 42 | 14 | 117 | 33 |
P60174 | Triosephosphate isomerase | 44 | 15 | 104 | 23 |
P29144 | Tripeptidyl-peptidase 2 | 1 | 1 | 78 | 45 |
P54578 | Ubiquitin carboxyl-terminal hydrolase 14 | 18 | 9 | 87 | 27 |
Q9C0C9 | Ubiquitin-conjugating enzyme E2 O | 5 | 3 | 77 | 37 |
Q96RL7 | Vacuolar protein sorting-associated protein 13A | 11 | 9 | ||
F5GYF5 | Vacuolar protein sorting-associated protein 35 | 1 | 1 | 14 | 11 |
Sorted populations of reticulocytes and extruded nuclei were fractionated by 1D SDS-PAGE and subjected to Nano LC mass spectrometry. An abridged list containing key cytosolic and endocytic proteins of interest is shown. Total peptide column is the total number of peptides (and therefore an indication of a particular protein’s abundance) detected in the population, whilst the unique peptide column indicates the number of unique peptides detected. To assess differences between nuclei and reticulocyte populations the total peptide number should be used.