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Antigen-specific precipitating antibodies to Aspergillus flavus and Penicillium digitatum in the patient’s serum (a) were found by Ouchterlony double immunodiffusion testing with A. flavus and P. digitatum antigens. We confirmed the presence of antigen-specific precipitating antibodies to P. digitatum by using antigen derived from the patient’s sputum culture fluid or extracted from the fungus in his sputum (b).
