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. 2013 Apr 2;8(4):e60591. doi: 10.1371/journal.pone.0060591

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© 2013 Boehme et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Artificial circulatory setup with two independent circuits filled with human packed red blood cells (haematocrit of 30%). A switching valve between the circuits enabled a step-change between highly oxygenated (circuit 1: purged with pure oxygen) and oxygen free blood (circuit 2: purged with nitrogen). Black arrows represent direction of blood flow. Adapting the settings of the rollerpumps and the heating-cooling device (heat exchanger) allowed blood-flow and temperature to be controlled. Via the O₂/N₂ blenders, oxygen content could be adapted at fixed sweep gas flow over the oxygenators. Measurement chamber contained 1.) ports for insertion of MFPF probes (Foxy-AL 300); 2.) a temperature probe and 3.) a sampling port for Clark-typed based (CTE) P_O2 analysis (ABL 700).