Table 1. Percent inhibition of Tat-transactivation after depleting anti-Tat antibodies and IgG fractions of the plasma.
profiles of response | No. | Tat | inhibition (%)a | inhibition (%)b | inhibition (%)c |
Full potential response | 1 | 3.216 | 74.01 | 27.59 | 43.85 |
2 | 1.166 | 76.83 | 30.87 | 39.63 | |
3 | 0.774 | 80.95 | 13.68 | 20.54 | |
Combined response(N preferred reaction) | 4 | 1.861 | 70.36 | 38.43 | 47.42 |
5 | 0.681 | 82.45 | 22.74 | 36.72 | |
6 | 0.476 | 78.60 | 17.25 | 15.37 | |
7 | 1.561 | 74.54 | 26.36 | 37.31 | |
9 | 1.706 | 72.19 | 19.14 | 38.95 | |
N-specific response | 17 | 0.264 | 70.28 | 11.31 | 17.29 |
18 | 0.271 | 68.27 | 16.95 | 13.28 | |
monoclonal Tat antibody | 2.518 | 75.25 | 12.58 | 11.95 | |
HIV+Tat- | 1 | 0.065 | 22.55 | 20.12 | 18.70 |
2 | 0.06 | 18.18 | 15.20 | 19.12 | |
3 | 0.051 | 9.41 | 8.57 | 7.31 | |
4 | 0.053 | 26.37 | 19.26 | 22.12 | |
5 | 0.058 | 17.66 | 15.33 | 13.15 | |
6 | 0.053 | 7.33 | 10.02 | 9.87 | |
7 | 0.054 | 15.79 | 11.78 | 9.96 | |
8 | 0.067 | 23.28 | 12.12 | 17.83 | |
9 | 0.063 | 13.75 | 14.00 | 12.26 | |
10 | 0.062 | 12.65 | 10.98 | 12.82 | |
HIV- | 1 | 0.087 | 11.47 | 12.42 | 13.16 |
2 | 0.076 | 18.59 | 10.78 | 17.89 | |
3 | 0.058 | 23.43 | 18.72 | 21.10 | |
4 | 0.071 | 8.39 | 10.80 | 7.89 | |
5 | 0.072 | 19.34 | 16.54 | 13.32 | |
6 | 0.071 | 3.23 | 5.33 | 7.88 | |
7 | 0.083 | 15.55 | 10.40 | 7.97 | |
8 | 0.064 | 24.93 | 15.87 | 16.89 | |
9 | 0.087 | 16.72 | 17.80 | 14.38 | |
10 | 0.067 | 9.78 | 11.20 | 7.72 |
All sera were diluted at 1:50 for Tat-neutralization assay. The ELISA OD values and inhibition of Tat-transactivation (inhibition (%)) are shown. “No.” represents the number of each plasma sample. a % inhibition of Tat-transactivation after depleting non-specific Tat antibodies with coated pPEPTIDE2 by ELISA. b % inhibition of Tat-transactivation after depleting Tat antibodies with coated Tat by ELISA. c % inhibition of Tat-transactivation after depleting IgG fractions with coated protein G by ELISA.