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. 2013 Apr 2;8(4):e60825. doi: 10.1371/journal.pone.0060825

Table 1. Percent inhibition of Tat-transactivation after depleting anti-Tat antibodies and IgG fractions of the plasma.

profiles of response No. Tat inhibition (%)a inhibition (%)b inhibition (%)c
Full potential response 1 3.216 74.01 27.59 43.85
2 1.166 76.83 30.87 39.63
3 0.774 80.95 13.68 20.54
Combined response(N preferred reaction) 4 1.861 70.36 38.43 47.42
5 0.681 82.45 22.74 36.72
6 0.476 78.60 17.25 15.37
7 1.561 74.54 26.36 37.31
9 1.706 72.19 19.14 38.95
N-specific response 17 0.264 70.28 11.31 17.29
18 0.271 68.27 16.95 13.28
monoclonal Tat antibody 2.518 75.25 12.58 11.95
HIV+Tat- 1 0.065 22.55 20.12 18.70
2 0.06 18.18 15.20 19.12
3 0.051 9.41 8.57 7.31
4 0.053 26.37 19.26 22.12
5 0.058 17.66 15.33 13.15
6 0.053 7.33 10.02 9.87
7 0.054 15.79 11.78 9.96
8 0.067 23.28 12.12 17.83
9 0.063 13.75 14.00 12.26
10 0.062 12.65 10.98 12.82
HIV- 1 0.087 11.47 12.42 13.16
2 0.076 18.59 10.78 17.89
3 0.058 23.43 18.72 21.10
4 0.071 8.39 10.80 7.89
5 0.072 19.34 16.54 13.32
6 0.071 3.23 5.33 7.88
7 0.083 15.55 10.40 7.97
8 0.064 24.93 15.87 16.89
9 0.087 16.72 17.80 14.38
10 0.067 9.78 11.20 7.72

All sera were diluted at 1:50 for Tat-neutralization assay. The ELISA OD values and inhibition of Tat-transactivation (inhibition (%)) are shown. “No.” represents the number of each plasma sample. a % inhibition of Tat-transactivation after depleting non-specific Tat antibodies with coated pPEPTIDE2 by ELISA. b % inhibition of Tat-transactivation after depleting Tat antibodies with coated Tat by ELISA. c % inhibition of Tat-transactivation after depleting IgG fractions with coated protein G by ELISA.