Table 2. Predicted coding sequences on plasmid pMAB01 and the putative functions.
Coding sequence | Protein size (aa) | GC content (%) | Putative function - best hit homolog in the database* | GenBank accession no. of the best hit | Identity to the closest homolog in the database |
trfA1 | 406 | 66 | Replication initiation protein - plasmid pB10 | NP_858039 | 100 |
ssb | 113 | 60 | Single-stranded DNA-binding protein - plasmid R751 | NP_044238 | 100 |
trbA | 120 | 59 | Conjugal transfer protein TrbA - plasmid pB10 | NP_857976 | 100 |
trbB | 320 | 62 | Conjugal transfer protein trbB - plasmid pB10 | NP_857977 | 100 |
trbC | 154 | 67 | Conjugal transfer protein TrbC - plasmid pB10 | NP_857978 | 100 |
trbD | 103 | 63 | Conjugal transfer protein TrbD - plasmid pB10 | NP_857979 | 100 |
trbE | 852 | 64 | Conjugal transfer protein TrbE - plasmid pB10 | NP_857980 | 100 |
trbF | 260 | 66 | Conjugal transfer protein TrbF - plasmid pB10 | NP_857981 | 100 |
trbG | 306 | 64 | Conjugal transfer protein TrbG - plasmid pB10 | NP_857982 | 100 |
trbH | 162 | 71 | Conjugal transfer protein TrbH - plasmid pB10 | NP_857983 | 100 |
trbI | 473 | 67 | Conjugal transfer protein TrbI - plasmid pB10 | NP_857984 | 100 |
trbJ | 254 | 61 | Conjugal transfer protein TrbJ - plasmid R751 | NP_044248 | 100 |
trbK | 75 | 62 | Conjugal transfer entry exclusion protein TrbK - plasmid pB10 | NP_857986 | 100 |
trbL | 571 | 68 | Conjugal transfer protein TrbL - plasmid pB10 | NP_857987 | 100 |
trbM | 195 | 68 | Conjugal transfer protein TrbM - plasmid pB10 | NP_857988 | 100 |
trbN | 211 | 69 | Conjugal transfer protein TrbN - plasmid pB10 | NP_857989 | 100 |
trbO | 88 | 64 | Conjugal transfer protein TrbO - plasmid R751 | NP_044253 | 100 |
trbP | 232 | 68 | Conjugal transfer protein TrbP - plasmid pB10 | NP_857991 | 100 |
pMAB01_019 | 143 | 66 | Outer membrane protein precursor - plasmid pB10 | NP_857992 | 100 |
orf-5 | 166 | 65 | GCN5-like N-acetyltransferase - plasmid R100 | NP_052894 | 100 |
sulI | 279 | 62 | Dihydropteroate synthase type-1 - plasmid pTET3 | NP_478074 | 100 |
qacEdelta1 | 115 | 50 | Ethidium bromide resistance protein - plasmid R100 | NP_052896 | 100 |
aac(6')-Ib | 172 | 54 | Aminoglycoside 6'-N-acetyltransferase - plasmid BRA100 | YP_006316013 | 100 |
pMAB01_024 | 134 | 54 | Glyoxalase-like domain protein - plasmid BRA100 | YP_006316014 | 100 |
intI | 337 | 61 | Class I integron integrase - plasmid pCTX-M3 | NP_775042 | 100 |
pMAB01_026 | 136 | 65 | Hypothetical protein - protein pRAx | YP_002995716 | 100 |
traC | 1448 | 69 | DNA primase TraC - plasmid pB10 | NP_857999 | 100 |
traD | 129 | 73 | Protein TraD - plasmid R751 | NP_044268 | 100 |
traE | 687 | 67 | DNA topoisomerase III - plasmid pB10 | NP_858001 | 100 |
traF | 178 | 66 | Plasmid transfer protein TraF - plasmid R751 | NP_044270 | 100 |
traG | 637 | 66 | Conjugal transfer protein TraG - plasmid pB10 | NP_858003 | 100 |
traI | 746 | 67 | DNA relaxase - plasmid pB10 | NP_858004 | 100 |
traH | 130 | 68 | Relaxosome stabilizing protein - plasmid R751 | NP_044273 | 100 |
traJ | 124 | 67 | Conjugal transfer relaxosome component TraJ - plasmid pB10 | NP_858006 | 100 |
traK | 132 | 63 | Conjugal transfer protein TraK - plasmid R751 | NP_044276 | 100 |
traL | 241 | 63 | Conjugal transfer protein TraL- plasmid pB10 | NP_858008 | 100 |
traM | 146 | 68 | Conjugal transfer protein TraM - plasmid R751 | NP_044278 | 100 |
traN | 217 | 69 | Protein TraN - plasmid pB10 | NP_858010 | 100 |
traO | 115 | 58 | Protein TraO - plasmid pB10 | NP_858011 | 100 |
kfrA | 343 | 75 | Protein KfrA - plasmid pB10 | NP_858012 | 100 |
korB | 349 | 67 | Transcriptional repressor protein KorB - plasmid pB10 | NP_858013 | 100 |
incC2 | 254 | 65 | Inclusion membrane protein IncC2 - plasmid pB10 | NP_858015 | 100 |
incC1 | 360 | 65 | Inclusion membrane protein IncC1 - plasmid pB10 | NP_858014 | 100 |
korA | 102 | 63 | Transcriptional repressor protein KorA - plasmid pB10 | NP_858016 | 100 |
kleF | 109 | 63 | Stable inheritance protein KleF - plasmid pB10 | NP_858017 | 100 |
kleE | 108 | 63 | Stable inheritance protein KleE - plasmid pB10 | NP_858018 | 100 |
kleA | 78 | 67 | Stable inheritance protein KleA - plasmid pB10 | NP_858019 | 100 |
korC | 85 | 68 | Transcriptional repressor protein KorC - plasmid pB10 | NP_858020 | 100 |
klcB | 402 | 70 | Stable inheritance protein KlcB - plasmid pB10 | NP_858021 | 100 |
pMAB01_050 | 87 | 68 | Hypothetical protein - plasmid pJP4 | YP_293608 | 100 |
klcA | 142 | 68 | Antirestriction protein KlcA - plasmid pB10 | NP_858022 | 100 |
din | 87 | 65 | Damage inducible-like protein - plasmid pB10 | NP_858023 | 100 |
relE | 93 | 68 | Plasmid stabilization system - plasmid pB10 | NP_858024 | 100 |
tnpA | 971 | 60 | Transposase for insertion sequence element IS1071 in transposon Tn5271 - plasmid pADP-1 | NP_862478 | 100 |
strA | 267 | 56 | Aminoglycoside resistance protein A - plasmid pIG1 | NP_054472 | 100 |
strB | 278 | 56 | Aminoglycoside resistance protein B - plasmid RSF1010 | NP_044302 | 100 |
tnpR | 186 | 62 | Transposon Tn21 resolvase - plasmid pB10 | NP_858031 | 100 |
orf-2 | 329 | 62 | Diguanylate phosphodiesterase - plasmid pB10 | NP_858032 | 100 |
merE | 78 | 65 | Mercuric resistance protein MerE - plasmid pB10 | NP_858033 | 100 |
merD | 121 | 70 | HTH-type transcriptional regulator MerD - plasmid pB10 | NP_858034 | 100 |
merA | 561 | 66 | Mercuric reductase - plasmid pB10 | NP_858035 | 100 |
merP | 91 | 62 | Mercuric transport protein periplasmic component - plasmid pB10 | NP_858036 | 100 |
merT | 116 | 62 | Mercuric transport protein - plasmid pJP4 | YP_025416 | 100 |
merR | 144 | 61 | Mercuric resistance operon regulatory protein - plasmid pB10 | NP_858038 | 100 |
: Certain CDSs showed more than one best hit, although the first one was selected to represent the homologous sequence.