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. 2013 Apr 2;8(4):e60746. doi: 10.1371/journal.pone.0060746

Table 2. Predicted coding sequences on plasmid pMAB01 and the putative functions.

Coding sequence Protein size (aa) GC content (%) Putative function - best hit homolog in the database* GenBank accession no. of the best hit Identity to the closest homolog in the database
trfA1 406 66 Replication initiation protein - plasmid pB10 NP_858039 100
ssb 113 60 Single-stranded DNA-binding protein - plasmid R751 NP_044238 100
trbA 120 59 Conjugal transfer protein TrbA - plasmid pB10 NP_857976 100
trbB 320 62 Conjugal transfer protein trbB - plasmid pB10 NP_857977 100
trbC 154 67 Conjugal transfer protein TrbC - plasmid pB10 NP_857978 100
trbD 103 63 Conjugal transfer protein TrbD - plasmid pB10 NP_857979 100
trbE 852 64 Conjugal transfer protein TrbE - plasmid pB10 NP_857980 100
trbF 260 66 Conjugal transfer protein TrbF - plasmid pB10 NP_857981 100
trbG 306 64 Conjugal transfer protein TrbG - plasmid pB10 NP_857982 100
trbH 162 71 Conjugal transfer protein TrbH - plasmid pB10 NP_857983 100
trbI 473 67 Conjugal transfer protein TrbI - plasmid pB10 NP_857984 100
trbJ 254 61 Conjugal transfer protein TrbJ - plasmid R751 NP_044248 100
trbK 75 62 Conjugal transfer entry exclusion protein TrbK - plasmid pB10 NP_857986 100
trbL 571 68 Conjugal transfer protein TrbL - plasmid pB10 NP_857987 100
trbM 195 68 Conjugal transfer protein TrbM - plasmid pB10 NP_857988 100
trbN 211 69 Conjugal transfer protein TrbN - plasmid pB10 NP_857989 100
trbO 88 64 Conjugal transfer protein TrbO - plasmid R751 NP_044253 100
trbP 232 68 Conjugal transfer protein TrbP - plasmid pB10 NP_857991 100
pMAB01_019 143 66 Outer membrane protein precursor - plasmid pB10 NP_857992 100
orf-5 166 65 GCN5-like N-acetyltransferase - plasmid R100 NP_052894 100
sulI 279 62 Dihydropteroate synthase type-1 - plasmid pTET3 NP_478074 100
qacEdelta1 115 50 Ethidium bromide resistance protein - plasmid R100 NP_052896 100
aac(6')-Ib 172 54 Aminoglycoside 6'-N-acetyltransferase - plasmid BRA100 YP_006316013 100
pMAB01_024 134 54 Glyoxalase-like domain protein - plasmid BRA100 YP_006316014 100
intI 337 61 Class I integron integrase - plasmid pCTX-M3 NP_775042 100
pMAB01_026 136 65 Hypothetical protein - protein pRAx YP_002995716 100
traC 1448 69 DNA primase TraC - plasmid pB10 NP_857999 100
traD 129 73 Protein TraD - plasmid R751 NP_044268 100
traE 687 67 DNA topoisomerase III - plasmid pB10 NP_858001 100
traF 178 66 Plasmid transfer protein TraF - plasmid R751 NP_044270 100
traG 637 66 Conjugal transfer protein TraG - plasmid pB10 NP_858003 100
traI 746 67 DNA relaxase - plasmid pB10 NP_858004 100
traH 130 68 Relaxosome stabilizing protein - plasmid R751 NP_044273 100
traJ 124 67 Conjugal transfer relaxosome component TraJ - plasmid pB10 NP_858006 100
traK 132 63 Conjugal transfer protein TraK - plasmid R751 NP_044276 100
traL 241 63 Conjugal transfer protein TraL- plasmid pB10 NP_858008 100
traM 146 68 Conjugal transfer protein TraM - plasmid R751 NP_044278 100
traN 217 69 Protein TraN - plasmid pB10 NP_858010 100
traO 115 58 Protein TraO - plasmid pB10 NP_858011 100
kfrA 343 75 Protein KfrA - plasmid pB10 NP_858012 100
korB 349 67 Transcriptional repressor protein KorB - plasmid pB10 NP_858013 100
incC2 254 65 Inclusion membrane protein IncC2 - plasmid pB10 NP_858015 100
incC1 360 65 Inclusion membrane protein IncC1 - plasmid pB10 NP_858014 100
korA 102 63 Transcriptional repressor protein KorA - plasmid pB10 NP_858016 100
kleF 109 63 Stable inheritance protein KleF - plasmid pB10 NP_858017 100
kleE 108 63 Stable inheritance protein KleE - plasmid pB10 NP_858018 100
kleA 78 67 Stable inheritance protein KleA - plasmid pB10 NP_858019 100
korC 85 68 Transcriptional repressor protein KorC - plasmid pB10 NP_858020 100
klcB 402 70 Stable inheritance protein KlcB - plasmid pB10 NP_858021 100
pMAB01_050 87 68 Hypothetical protein - plasmid pJP4 YP_293608 100
klcA 142 68 Antirestriction protein KlcA - plasmid pB10 NP_858022 100
din 87 65 Damage inducible-like protein - plasmid pB10 NP_858023 100
relE 93 68 Plasmid stabilization system - plasmid pB10 NP_858024 100
tnpA 971 60 Transposase for insertion sequence element IS1071 in transposon Tn5271 - plasmid pADP-1 NP_862478 100
strA 267 56 Aminoglycoside resistance protein A - plasmid pIG1 NP_054472 100
strB 278 56 Aminoglycoside resistance protein B - plasmid RSF1010 NP_044302 100
tnpR 186 62 Transposon Tn21 resolvase - plasmid pB10 NP_858031 100
orf-2 329 62 Diguanylate phosphodiesterase - plasmid pB10 NP_858032 100
merE 78 65 Mercuric resistance protein MerE - plasmid pB10 NP_858033 100
merD 121 70 HTH-type transcriptional regulator MerD - plasmid pB10 NP_858034 100
merA 561 66 Mercuric reductase - plasmid pB10 NP_858035 100
merP 91 62 Mercuric transport protein periplasmic component - plasmid pB10 NP_858036 100
merT 116 62 Mercuric transport protein - plasmid pJP4 YP_025416 100
merR 144 61 Mercuric resistance operon regulatory protein - plasmid pB10 NP_858038 100
*

: Certain CDSs showed more than one best hit, although the first one was selected to represent the homologous sequence.