Table 3. Detection of pMAB01 plasmid sequences in Mycobacterium abscessus subsp. bolletii clinical isolates and type strains.
| Isolates | N | PCR (pMAB01) | PFGE-DraI (∼50 kb band) | PFGE-DraI (< 50 kb band) | Hybridization (trfA probe) |
| Surgical isolates (epidemic | 9 | + | + | + | |
| strain) | 3 | + | + | + | |
| 1 | + | – | +* | ||
| 2 | – | – | – | ||
| Non-surgical isolates (epidemic | 3 | + | + | + | |
| strain) | 8 | – | – | – | |
| Other strains | 13 | – | – | ND | |
| Type strains** | 2 | – | – | – | |
| Total | 41 |
N: Number of isolates
+: Positive result
–: Negative result
ND: not determined
*
: Near gel origin or between the 50 and 100-kb molecular markers (two different experiments with the same isolate)
**
: M. abscessus ATCC 19977 and M. massiliense CCUG 48898