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. 2013 Apr 2;8(4):e60307. doi: 10.1371/journal.pone.0060307

Figure 1. Identification of plasmodial DNA in Fayum mummies.

Figure 1

(a) Amplification of partial apical membrane antigen 1 gene (AMA1). Lane M, 100- bp DNA ladder (NEB); lane 1, 1543; lane 6, 1554; lane 9, 1564; lane 11, 1622; lane 14, 1643; lane 15, PCR negative control; (b) Amplification of MSP1 K1 allele. Lane M, 100- bp DNA ladder (NEB); lane 1, 1543; lane 2, 1554; lane 3, 1554; lane 4, 1622; lane 5, 1622; lane 6, 1564; lane 7, 1611; lane 8, 1643; lane 9, PCR negative control; (c) Nucleotide alignment of Plasmodium MSP1 K1 alleles amplified from mummy 1564 in comparison with Plasmodium falciparum isolate (accession no. AF061126). The variable central region of K1 allele types differ by the number and arrangement of repeat motifs. A total of 8 K1 type alleles were identified. Dashes represent gaps introduced to maximize the alignment.