Table 1. Mass spectrometry results of the unmodified and modified heavy chains (HC).
Antibody | Ligand | Calculated Mass [Da] | Observed Mass [Da] |
chCE7degl | No ligand | 49362 | 49362 |
(DOTA)-decalysine (1684 Da) | 51029 | 51029 | |
(DOTA)-decalysine+Potassium | 51066 | 51066 | |
(DOTA)3-decalysine (2457 Da) | 51802 | 51802 | |
(DOTA)3-decalysine+Potassium | 51839 | 51837 | |
chCE7agl | No ligand | 49374 | 49374 |
(DOTA)-decalysine (1684 Da) | 51024 | 51025 | |
(DOTA)-decalysine+Potassium | 51061 | 51061 | |
(DOTA)3-decalysine (2457 Da) | 51797 | 51798 | |
(DOTA)3-decalysine+Potassium | 51834 | 51833 |