Figure 1. Schematic representation of the DNA E1 region used to express in MDCK-E1 transcomplementing cells and the predicted major mRNA and protein derived from E1A and E1B genes.

Predicted mRNAs and proteins were inferred by sequence homology [26] with human adenovirus. E1A 32 kDa and 28 kDa are translated from 13S and 12S mRNAs from E1A respectively. E1B 55 kDa is translated from 22S mRNA and E1B 19 kDa protein can be translated from the 22S or 13S mRNAs from E1B. Grey dashes underlying the mRNA products represent the generated PCR amplicon in the expression analysis of E1A and E1B genes. One set of primers was designed to assess E1A expression since it is expected that most generated splicing transcripts will contain the initial 5′ region of exon 1. Two sets of primers were design for E1B to confirm the formation of mRNA transcripts containing the 55 KDa open reading frames.