Table 2. Amplification of CAVGFP and JBΔ5 in Cre-expressing clones and the corresponding parental cells.
| Cells | Viral vectors | ||||
| CAVGFP | JBΔ5 | ||||
| Amplification ratio | Productivity ratio | Amplification ratio | Fold decrease | Excision efficiency (%) | |
| MDCK-E1#106 | 688±172 | – | 605±151 | – | – |
| MDCK-E1#106-Cre#2 | 537±134 | 0.8 | 22±6 | 28 | 96 |
| MDCK-E1#106-Cre#10 | 928±232 | 1.3 | 3±1 | 201 | 99 |
| MDCK-E1#106-Cre#19 | 590±148 | 0.9 | 22±6 | 28 | 96 |
| MDCK-E1#106-Cre#26 | 798±200 | 1.2 | 22±6 | 28 | 96 |
| MDCK-E1#106-Cre#30 | 882±221 | 1.3 | 25±6 | 24 | 96 |
| DKZeo | 475±106 | – | 299±75 | – | – |
| DKCre | 604±151 | 1.3 | 10±3 | 30 | 97 |
The error in amplification ratio corresponds to 25% inter-assays variability. The productivity ratio corresponds to the ratio between CAVGFP amplification using Cre expressing and corresponding parental cells. Fold decrease in JBΔ5 production was calculated using the ratio between amplification value of parental cells (MDCK-E1#106 and DKZeo) and the corresponding Cre-expressing cells. Excision efficiency was calculated assuming that the difference in the amplification ratio of parental and corresponding Cre-expressing cells corresponds to unpackaged viral genomes.