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. 2013 Apr 2;8(4):e60286. doi: 10.1371/journal.pone.0060286

Figure 3. Sevoflurane increased the amplitude and event rate of GABAergic miniature IPSCs.

Figure 3

(A) Representative recording of spontaneous GABAA receptor-mediated IPSCs (upper trace). To focus on postsynaptic sevoflurane effects miniature IPSCs (mIPSCs) were isolated by adding 1 µM tetrodotoxin (TTX, middle trace). Subsequently, 1.5 MAC sevoflurane was applied (lower trace). (B) Pooled data revealed an event rate of 22.9±7.0 Hz for control condition (n = 9). In 5 out of 9 experiments, no single mIPSC could be detected after TTX application. In 4 experiments, the frequency of mIPSC was less than 99% compared to the initial spontaneous event rate without TTX (mIPSC frequency 0.046±0.018 Hz (n = 4). Additional application of 1.5 MAC sevoflurane increased the frequency of mIPSC events to 0.33±0.12 Hz (n = 4). (C) Sevoflurane prolonged the mean decay time of mIPSCs (TTX condition 28.0±1.4 ms vs. 40.9±4.8 ms sevoflurane, n = 4, p<0.05). (D) Additionally, the mean of mIPSC amplitudes augmented from 37.3±4.1 pA (TTX condition) to 50.0±2.1 pA (n = 4, p<0.05).