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. 2013 Apr 2;8(4):e60151. doi: 10.1371/journal.pone.0060151

Figure 1. Strategy for Duplex SmartAmp-based detection of SNP (c.309T>G) in the MDM2 gene.

Figure 1

A: The MDM2 gene is located on chromosome 12q14.3-q15, where SNP c.309T>G resides in intron 1. Annealing sites of the TP, FP, BP, OP, nBP, and oBP primers are shown in this schematic illustration. The size of each annealing site is numerically indicated in base (b) units. nBP, oBP(WT), and oBP(SNP) are designed to compete with each other to anneal with a site proximal to the SNP position. B: Horizontal arrows indicate annealing sites of primers. SNP (c.309T>G) is indicated by a vertical arrow.