Table 1. Sequences of primers developed for the Duplex SmartAmp method.

Primer	5′-DNA sequence-3′
TP	CGCGGGAGGTC AGCGTTCACACTAGTGACCC
FP	ACCTTCTATACCCTCAGAAGGT CGGGAGTTCAGGGTAAAGGT
BP	TCGCAGGTGCCTGTC
nBP	GGCTGCGGGGCC
OP	CAATCCCGCCCAGACTAC
oBP (WT)	5′- CGGGGCCGCZTC - 3′ (Z : Thymine labeled with thiazole pink)
oBP(SNP)	5′- CGGGGUCCGCTGC - 3′ (U : Thymine labeled with thiazole orange)

TP: The turn-back region is underlined.

FP: The folding region is underlined.

“Z” and “U” indicate thiazole pink- and thiazole orange-labeled thymines in oBP, respectively.