Table 2. Comparison of results obtained by the Duplex SmartAmp and PCR-RFLP methods.
Genomic DNA Samples : Duplex SmartAmp | Blood Samples : Duplex SmartAmp | ||||||
T/T | T/G | G/G | T/T | T/G | G/G | ||
PCR-RFLP | T/T | 25 | 0 | 0 | 4 | 0 | 0 |
T/G | 0 | 46 | 0 | 0 | 15 | 0 | |
G/G | 0 | 0 | 25 | 0 | 0 | 5 |
A total of 96 genomic DNA samples and 24 blood samples were subjected to the Duplex SmartAmp and PCR-RFLP assay methods to examine the genotypes of SNP (c.309T>G) in the MDM2 gene.