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. 2013 Apr 2;8(4):e60453. doi: 10.1371/journal.pone.0060453

Figure 1. Kinetics of chemokine receptor expression by CD11b+ DCs.

Figure 1

Mice were nasally immunized weekly for three consecutive weeks with OVA plus Ad-FL (A-D). Five, 10, 14 and 21 days after the initial immunization, mononuclear cells (MNCs) from NALT (A), CLNs (B), NPs (C), and SMGs (D) were stained with FITC-conjugated anti-CD11b, PE-labeled anti-CCR5, allophycocyanin-tagged anti-CCR6 or -CCR7 and biotinylated anti-CD11c mAbs followed by PerCP-Cy5.5-streptavidin. CD11c+ and CD11b+ cells were gated and their CCR expression was analyzed by FACSCalibur®. The values shown are the mean of the actual numbers of cells ± SEM taken from five separate experiments (two samples/experiment) with a total of 10 samples in each experimental group. * p<0.05, ** p<0.01 when compared with day 0.