Skip to main content
. 2013 Apr 2;8(4):e60453. doi: 10.1371/journal.pone.0060453

Figure 2. CCL3, CCL4, CCL5 and CCL20 synthesis in various mucosal tissues.

Figure 2

Mice were nasally immunized weekly for either two or three consecutive weeks with OVA plus Ad-FL. Three days after the second immunization (day 10) or one week after the last immunization (day 21), MNCs were taken from NALT, NPs and SMGs, and cultured in vitro for 5 days. Culture supernatants were harvested after 5 days of incubation and analyzed for the respective chemokine by ELISA. The values shown are the mean ± SEM taken from five separate experiments with a total of 25 mice in each experimental group. * p<0.05, ** p<0.01 when compared with naïve mice (day 0).