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DMQ0 binding perturbs the oxidized diiron center in GB1-hCLK-1 as revealed by Mössbauer spectroscopy. (a) Mössbauer spectrum of 1 mM oxidized GB1-hCLK-1 without DMQ0. (b) Mössbauer spectrum of 1 mM oxidized GB1-hCLK-1 in the presence of 1.6 mM DMQ0. The red lines are simulated spectra of the components, which combine as indicated by the blue line overlaid on the experimental spectrum.
