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GB1-hCLK-1 catalyzes the hydroxylation of DMQ0. The product generated in the reaction of 5 μM GB1-hCLK-1, 1 mM DMQ0 and 1 mM NADH is identified by GC-MS. (a) GC-MS chromatograph of DMQ0 (top), reaction product (middle), and DMQ0-OH (bottom). (b) MS spectra of DMQ0 (top) and reaction product, DMQ0-OH (bottom).
