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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Biomol NMR. 2013 Jan 12;55(3):249–256. doi: 10.1007/s10858-013-9706-1

Scheme 1.

Scheme 1

Hydrogen exchange effect during the 15N R2 relaxation time period. The H/H exchange rate is QkHX and the H/D exchange rate is (1-Q)kHX when the H2O content in the sample solvent is Q and the D2O content is (1-Q). In H/H exchange, the product NH′ is distinguished from the reactant NH.