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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Cytokine. 2013 Mar 13;62(1):91–95. doi: 10.1016/j.cyto.2013.02.010

Fig. 1.

Fig. 1

Th1- and Th2-inducing conditions lead to highly polarized IFN-γ expression and IL-4 expression, respectively. Naïve CD4+ T cells were not primed or primed under neutralized conditions [indicated as Neu, these conditions contained anti-IFN-γ antibody (10 μg/ml) and anti-IL-12 antibody (10 μg/ml)], Th1-inducing conditions [indicated as Th1), these conditions contained IFN-γ (20 ng/ml) plus anti-IL-4 antibody (10 μg/ml)] or Th2-inducing conditions [indicated as Th2, these conditions contained IL-4 (5 ng/ml) plus anti-IFN-γ antibody (10 μg/ml) and anti-IL-12 antibody (10 μg/ml)] for 7 days. IL-4 and IFN-γ protein expression was analyzed by intracellular staining. The numbers inside the FACS plots indicate the positive percentage within the gated CD4+ T cells.