Fig. 2.

IFN-γ suppresses H3K4me2 modification in the IE region of the Il4 gene. Naïve CD4⁺ T cells from C57BL/6 mice were primed under neutralized conditions, Th1-inducing conditions or Th2-inducing conditions for either 2 or 4 days. The resultant cells were harvested, cross-linked, and sonicated. H3K4me2 modification was measured by immunoprecipitation with anti-dimethylated lysine 4 residue antibody. The amount of immunoprecipitated IE fragment was measured by Real-Time PCR and shown as the percentage of input DNA. Values represent mean ± SD of triplicates. Ns= not significant. Data from one experiment represent two independent experiments with similar results.