Fig. 3.

IFN-γ directly suppresses H3K4me2 modification. Naïve CD4⁺ T cells from C57BL/6 and IFNγR^−/− mice were cultured for 7 days under the conditions indicated. The resultant cells were harvested, cross-linked, and sonicated. H3K4me2 modification was measured by immunoprecipitation with anti-dimethylated lysine 4 residue antibody. The amount of immunoprecipitated IE fragment was measured by Real-Time PCR and shown as the percentage of input DNA. Values represent mean ± SD of duplicates. Data from one experiment represent two independent experiments with similar finding.