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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Cytokine. 2013 Mar 13;62(1):91–95. doi: 10.1016/j.cyto.2013.02.010

Fig. 4.

Fig. 4

IFN-γ depends on STAT1 to suppress H3K4me2 modification in the IE region but not the silencer region. (A) Naïve CD4+ T cells from C57BL/6 and STAT1−/− mice were cultured for 7 days under the conditions indicated. The resultant cells were harvested, cross-linked, and sonicated. H3K4me2 modification was measured by immunoprecipitation with anti-dimethylated lysine 4 residue antibody. The amount of immunoprecipitated IE fragment was measured by Real-Time PCR and shown as the percentage of input DNA. (B) The amount of immunoprecipitated silencer fragment was measured Real-Time PCR. Values represent mean ± SD of triplicates. Data from one experiment represent two independent experiments with similar results.