Figure 3. Identification of CG3363 as ocm.

A. Genomic region near 60C showing predicted genes and five BACs used to rescue ocm. The BACs shown in black failed to rescue ocm mutations, but 117N13 (yellow) rescued viability and fertility of multiple ocm alleles. B. Conceptual translation of ocm reveals a protein with distinct motifs conserved between other Drosophila species (colored boxes) separated by diverged regions (thin line). The hatched box indicates the segment of OCM used to raise antibodies. C. Similar coding regions are found in the genomes of some other insects. The Bombyx alignment is taken from genomic DNA lacking cDNA support, so the exact alignment is uncertain. D. The Cys431 region (red) is also found in chordate Mga. E. Several Daphnia proteins contain Cys431 motifs. A second cys-rich region (orange) is also present in two of the Daphnia proteins.