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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Exp Eye Res. 2013 Jan 18;109:8–16. doi: 10.1016/j.exer.2013.01.005

Figure 2. GFP expression pattern after 20 and 40 min intracameral infusion of adenovirus encoding GFP.

Figure 2

Ad.cmv.gfp (5×105 particles) in 10 µl PBS were infused into mouse anterior chamber over 20 minutes (0.5 µl/min) or 40 minutes (0.25 µl/min). Three days after infusion, mouse eyes were collected and fixed. Anterior segments were flat mounted and the GFP expression was visualized and captured (2.5× amplification) using a fluorescence microscope. The images are representative results of four to five individual experiments for each condition using both CD1 and C57BL/6 mice (8–15 weeks old).