Mouse eyes were infused with (0, 3, 5, 7 and 10 µl) of PBS over 3 minutes. Three days after infusions, mouse eyes were collected and fixed with 2% paraformaldehyde + 2% glutaraldehyde. Mouse anterior segments were dissected, processed and sectioned in sagittal plane (semi-thin, 0.5 µm) through angle structures. The images were visualized and captured with a light microscope. Images are representative results of three individual experiments for each condition using C57BL/6 mice (10–22 weeks old).