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. Author manuscript; available in PMC: 2014 Mar 28.
Published in final edited form as: Mol Cell. 2013 Feb 28;49(6):1121–1133. doi: 10.1016/j.molcel.2013.01.034

Figure 2. Subunit Requirement for H3K4 Methylation Activity of the Yeast Set1 Complex.

Figure 2

(A) SDS-PAGE/Coomassie Blue staining and immunoblot analyses of purified ySet1Cs reconstituted with baculovirus vectors in the absence of the indicated subunits. Complex loading was normalized to FLAG-Set1 (except lane 3, see below).

(B and C) Free histone H3 (B) and H2Bub-chromatin templates (C) were subjected to in vitro HMT assays with indicated ySet1Cs.

Note that because of inefficient complex formation in the absence of Swd1, a volume of the Swd1-deficient sample (lanes 3) equal to that of the intact complex sample (lanes 1) was used for SDS-PAGE and in vitro HMT assays.

See also Figure S2.