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. 2013 Jan 14;43(3):705–715. doi: 10.1002/eji.201242794

Figure 3.

Figure 3

ICOS−/− mice show impaired expansion of Helios−Foxp3+ Treg cells within the LP. WT (circles) and ICOS−/− (squares) mice were infected with H. polygyrus and the expression of Foxp3, CD103, CD25, PD-1 and Helios by LP CD4+ T cells assessed at day 7 pi. (A) Representative staining for Foxp3 and Helios on CD4+ T cells. (B) Percentage of LP CD4+ T cells expressing Foxp3 was determined by flow cytometry. (C–E) Geometric mean expression intensity of (C) CD103, (D) CD25 and (E) PD-1 on CD4+Foxp3+ Treg cells. (F and G) Percentage of (F) Helios+Foxp3+ and (G) Helios−Foxp3+ Treg cells is shown. Data shown are from (B, F, G) four independent experiments or from (C, D, E) one experiment representative of three performed. Open symbols represent naïve mice and closed symbols represent infected WT mice. *p < 0.05 (Tukey HSD, ANOVA performed using combined data from three or four experiments).