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. 2013 Apr 3;4:59. doi: 10.3389/fphys.2013.00059

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Copyright © 2013 Ito, Kobayashi, Nakamura, Miyagi, Esaki, Hoshijima and Hirose.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Figure A2 — Specificity of antisera. Recognition of the antigens by the corresponding antisera demonstrated by immunoprecipitation. CA2a (lanes 1 and 2) and TlCA15-FLAG (lanes 3 and 4) were translated in vitro in the presence of [³⁵S]methionine and [³⁵S]cysteine. The labeled proteins were subjected to immunoprecipitation with anti-CA2a antiserum (lane 1, Ab), anti-TuCA15a antiserum (lane 3, Ab), or corresponding preimmune serum (lanes 2 and 4, Pre). The immunoprecitates were analyzed by SDS–PAGE followed by autoradiography. Arrow indicates the position of the band corresponding to TlCA15-FLAG, indicating crossreactivity of anti-TuCA15a with TlCA15a.