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. Author manuscript; available in PMC: 2014 Mar 28.
Published in final edited form as: Cell. 2013 Mar 28;153(1):216–227. doi: 10.1016/j.cell.2013.02.047

Figure 2.

Figure 2

Gαq-stimulated hypertrophy in NRVMs is blocked by PLCε siRNA or disruption of nuclear envelope scaffolding via mAKAP. (A) NRVMs were transduced with 50 MOI of adenovirus expressing wild type Gαq or LacZ. Cells were co-transduced with either PLCε siRNA or control scrambled siRNA (Ctl) adenovirus. After 48 hours cells were fixed, permeabilized and stained for α-actinin. Cell area was calculated from 200 cells each treatment with NIH Image J and pooled from 3 separate experiments in the right panel (+/− SEM). (B) Same as A except ANF/GAPDH ratio was determined by quantitative real time PCR (+/− SEM). (C) NRVMs were cotransduced with adenoviruses expressing Gαq and mAKAP-SR domain. ANF/GAPDH ratio was measured after 48 h (+/− SEM). All experiments were repeated 3 times and were analyzed by One way ANOVA, *p<0.05; ***p<0.005.