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. Author manuscript; available in PMC: 2013 Apr 3.
Published in final edited form as: Cell Cycle. 2010 Oct 9;9(19):4005–4012. doi: 10.4161/cc.9.19.13299

Figure 3.

Figure 3

L37 depletion does not result in nucleolar breakdown. (A) U2OS cells were transfected with control or L37 siRNAs for 72 h (left) or treated with nutlin for 72 h or with actinomycin D for 16 h (right). NpM expression was detected by immunofluorescence using a polyclonal antibody against B23 (top). Histograms showing the number of nucleoli per cellular nucleus are shown (bottom). (B) U2OS cells were interfered with the indicated siRNAs, and the levels of the indicated proteins were determined 72 h later by immunoblotting. (C) U2OS cells were interfered with the indicated siRNAs, and 72 h later mRNA abundance was quantified by qRt-pCR. Data are shown as means ± SD of three independent experiments assayed in triplicate. the Ct values were corrected by β-actin and fold expression values are relative to control siRNA. ***Student’s t-test, p < 0.001.