Figure 6. OsMADS57 interaction with OsTB1.
(a,b) Yeast two-hybrid assay. Diagrams represent the proteins. Yeast strains were grown on synthetic dropout medium lacking Trp, Leu, His and Ade, and stained for β-galactosidase activity. (c) Co-IP assay in Arabidopsis protoplasts. OsMADS57C, C-terminal 169–241 amino acids. (d) Effect of OsTB1 and OsTB1A159QWA162A163 (OsTB1m) on transcriptional regulation of D14 via OsMADS57 in protoplasts. (e) Model of the OsMADS57-mediated network for control of tillering. WB, western blot.