Table 1. The effects of DTT on deubiquitinases.
| No activity detected | Activated by DTT | Enhanced by DTT | ||
|---|---|---|---|---|
| USP1 | USP52 | USP3 | USP19 | USP5 |
| USP13 | USP53 | USP4 | USP21 | USP7 |
| USP14 | JOSD2 | USP8 | USP25 | CYLD |
| USP22 | YOD1 | USP10 | USP26 | UCHL1 |
| USP28 | OTUD7B | USP11 | USP29 | UCHL3 |
| USP44 | A20 | USP15 | USP37 | UCHL5 |
| USP48 | VCPIP1 | USP16 | BAP1 | |
The DUBs analysed can be grouped into three classes based on their response to DTT. (1) No activity detected: the DUBs do not have detectable activity even in the presence of DTT. (2) Activated by DTT: the DUBs have little activities by themselves, but can be activated by DTT. (3) Enhanced by DTT: the DUBs are active, but the activities are further enhanced by DTT.