Skip to main content
. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Neurosci. 2013 Mar 3;16(4):441–448. doi: 10.1038/nn.3351

Fig. 5. Restoring actin polymerization rescues the impaired L-LTP and contextual LTM caused by mTORC2 deficiency.

Fig. 5

a) Western blots show that jasplakinolide (JPK; 50 nM) increased the low F-actin/G-actin ratio in CA1 slices from rictor fb-KO mice (n=4 per group, t=3.821, *p<0.05) but not in control slices (n=4 per group, t=0.253, p=0.157). b) The same concentration of JPK restored L-LTP in rictor fb-KO slices (n=7 per group, LTP at 30 min, vehicle 67 ± 7.7%, JPK 73 ± 11.3%, H=0.0667, ANOVA on Ranks, p=0.852; LTP at 220 min, vehicle 23 ± 4.9%, JPK 73 ± 12.5%, F(1, 12)=9.81, p<0.01) but had no effect on L-LTP in WT slices (c; n=7 per group, LTP at 30 min: vehicle 74 ± 9.4%, JPK 72 ± 11.4%, F(1, 12)=0.989, p=0.784; LTP at 220 min: vehicle 64 ± 8.7%, JPK 68 ± 14.2%; F(1, 12)=0.010, p=0.921). The actin polymerization inhibitor Cytochalasin-D (Cyt-D; 100 nM) blocked L-LTP in WT slices (c; at 220 min 26 ± 7.8%, F(1,12)=9.215, p<0.01) but had no effect in rictor fb-KO slices (b, at 220 min 21 ± 7.9%, F(1, 13)=0.163, p=0.694). Insets in b and c are superimposed traces recorded before and 220 min after tetani. Calibration: 5 ms, 2 mV. d-e) Bilateral infusion of JPK (50 ng) into the dorsal hippocampus of rictor fb-KO mice (n=8 per group), immediately after a strong training protocol (two pairings of a tone with a 0.7 mA foot-shock, 2s), boosted contextual LTM (d; F(1, 14)=4.827, *p<0.05) but not auditory LTM (e; F(1, 14)=0.0407, p=0.843). Freezing was assessed 24 hr after training, as described in Fig. 2. f) In WT mice (n=8 per group) JPK bilateral infusion (50 ng) had no effect on contextual LTM (F(1, 14)=0.129, p=0.726). g) A single tetanic train elicits only E-LTP in vehicle-treated slices but a sustained L-LTP in JPK-treated slices (n=7 for vehicle, n=8 for JPK; LTP at 180 min: vehicle 19 ± 5.2%, JPK 81 ± 14.5%, ANOVA on Ranks H=10.59; p<0.001). Inset in g are superimposed single traces recorded before and 180 min after tetani. Calibration: 5 ms, 2 mV. h) Intra-hippocampal infusion of JPK immediately after a weak training (a single pairing of a tone with a 1s, 0.7 mA foot-shock) enhanced contextual fear LTM (n=15 for vehicle and n=16 for JPK; F(1, 29)=4.320, *p<0.05) but not contextual fear STM (i, n=9 for vehicle and n=10 for JPK, H=0.00167, ANOVA on Ranks, p=0.967).