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. Author manuscript; available in PMC: 2013 Jul 6.
Published in final edited form as: Cell. 2012 Jul 6;150(1):111–121. doi: 10.1016/j.cell.2012.04.044

Figure 3. eIF5B Is Required for the Accumulation of 40S Assembly Factors in 80S-like Ribosomes.

Figure 3

10–50% sucrose gradients from cell lysates from ΔeIF5B,Gal1::Fap7 cells grown in glucose for 24h. Longer times for Fap7 depletion were required because due to the absence of eIF5B the strain grows slowly even in galactose.

(A) Absorbance profile at 254 nm.

(B) Western blots for assembly factors.

(C) Northern blots for rRNAs and precursors.

See Figure S2 for related data.